Macromolecular Crystallography Protocols : Volume 1: Preparation And Crystallization of Macromolecules, Hardcover by Doublie, Sylvie (EDT), ISBN 1588292924, ISBN-13 9781588292926, Brand New, Free shipping in the US

Doublié (microbiology and molecular genetics, U. of Vermont) presents the first of two volumes dedicated to the description of laboratory protocols for macromolecular crystallography. The 13 chapters in this volume, as the subtitle indicates, are concerned with preparation and crystallization of macromolecules. The first five chapters examine the production of proteins for structural biology, specifically discussing the use of a dual affinity tag for the production of recombinant protein; the cloning, expression, and purification of yeast proteins in a medium-sized structural genomics group, baculovirus expression of integral membrane proteins, and selenomethionine substitution in bacteria and eukaryotic cells. Remaining chapters focus on crystallization and include such topics as dynamic light scattering, screening and optimization methods, the improvement of marginal crystals, high-throughput optimization, crystallization of membrane proteins, and methods for growing protein-DNA and protein-RNA crystals. The second volume explores structure determination. Annotation ©2007 Book News, Inc., Portland, OR ()