Fehling’s test is one of the sensitive tests for detection of reducing sugars. Fehling’s reagents comprise of two solution Fehling’s solution A and solution B. Fehling’s solution A is aqueous copper sulphate and Fehling’s solution B is alkaline sodium potassium tartarate (Rochelle salt). Rochelle salts (sodium potassium tartarate) present in the reagent acts as the chelating agent in this reaction. These two solutions are mixed in equal amount before test.

On heating an aldehyde or reducing sugar with Fehling’s solution give reddish brown precipitate. Formation of red precipitate of cuprous oxide denotes the presence of reducing sugar.

Dawn Scientific’s new Innovative series of cUSP Test Solutions. We offer Fehling’s solution A and solution B in a 500mL bottle. Our products are highest in quality and perfect for all your scientific research and experiments, used in Industrial, Educational and Laboratory settings.  

 ·       500 ml HDPE bottles of Each Fehling A and B solution

·       Chemical solution for general purpose, Educational, Industrial and Scientific Laboratory

·       Each bottle has safe handling & storage procedures printed directly on the bottle.

·       Concentration, Lot Number and expiry date Printed on the label

·       SDS available on request

·       This is a high-quality chemical product manufactured in the United States by Iso 9001:2015 certified Company

For Laboratory Use Only. Not for drug, food or household use. Keep out of reach of children. For more information, refer to the Safety Data Sheet.

Procedure of Fehling’s test:

1.    Take 1ml of sample in dry test tube.

2.    Take 1ml of distilled water in another tube as control.

3.    Add 1ml of Fehling’s reagent (A and B) to all the tubes.

4.    Keep in boiling water bath.

5.    Look for the development of red precipitate.

Result interpretation:

§  Positive Fehling’s test: reddish brown ppt (glucose, fructose, lactose)

§  Negative Fehling’s test: No red ppt (sucrose, starch)