Chu N6 medium | W/WO Vitamins

   -Chu N6 Basal Salt Mixture, W/O Vitamins

   -Chu N6 Medium, With Vitamins

Fine powder, applied for 50/100L media preparation, listed price is for 50L with Vitamins

Applications

For use in plant cell culture, defined to improve the formation, growth and differentiation of pollen callus in rice & cereal crops.

Components, mg per litre

  Total concentration Micro and Macro elements including -vitamins: 3956.53 mg/l

Preparation

Suspend 3.95g of the Chu N6 medium W/O Vitamins (3.95~3.96g Chu N6 medium With Vitamins) into distilled water^, Mix well and make up to 1000ml. Heat with continuous stirring,  Adjust the medium to the desired pH using 1M HCl/NaOH and aliquot into tubes or flasks. Sterilise in the autoclave at 121°C for 15 minutes (the media with Sucrose should be autoclaved at 115°C for 20 minutes or might turn dark at over 115°C).

^ During preparation, avoid naked flames and do not mix the basal salt mixture with reducing substances such as sucrose in powder form (they must be dissolved in liquid in sequence when preparing).

Storage

Media powder is extremely hygroscopic and should be protected from atmospheric moisture. If possible, the unused powder should be stored in a sealed box with silica desiccant and refrigerated at 2-8°C.

* Please select the product via the 'Media Type' drop-down box and purchase

** To request a quote and order a customised version, please contact support@gmexpression.com.

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