GENERAL MOLECULAR EXPRESSION SERVICE PTY LTD
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Schenk & Hildebrandt Medium W/WO Vitamins
- Schenk & Hildebrandt Basal Salt Mixture, W/O Vitamins
- Schenk & Hildebrandt Medium, With Vitamins
Fine powder, applied for 50/100L media preparation, listed price is for 50L with Vitamins
Applications
For use in plant cell culture and micropropagation.
Components, mg per litre
Micro Elements | |
CoCl2.6H2O | 0.1 |
CuSO4.5H2O | 0.2 |
FeNaEDTA | 19.8 |
H3BO4 | 5.0 |
KI | 1.0 |
MnSO4.H2O | 10.0 |
Na2MoO4.2H2O | 0.1 |
ZnSO4.7H2O | 1.0 |
Macro Elements | |
CaCl2 | 151.0 |
KNO3 | 2500.0 |
MgSO4 | 195.05 |
(NH4)H2PO4 | 300.0 |
Vitamins* | |
Myo-inositol | 1000.0 |
Nicotinic acid | 5.0 |
Pyridoxine HCl | 0.5 |
Thiamine HCl | 5.0 |
The total concentration of Micro and Macro elements including vitamins: 4193.75mg/l
Auxins (Optional)** | |
2,4-D # | 0.5 |
4-CPA# | 2.0 |
Cytokin## | 0.1 |
Kinetin## | 0.1 |
# For monocotyledonous cell
## For dicotyledonous cell
Sucrose & Agar (Optional)** | |
Sucrose | 20000 |
Agar | A.C.D. |
Element deletion | A.C.D. |
A.C.D.~ As Customers' Demands
Preparation
Suspend 3.18g of the S&H medium WO Vitamins(4.19g S&H medium with Vitamins) into distilled water ^, Mix well and make up to 1000ml. Heat with continuous stirring, Adjust the medium to the desired pH using 1M HCl/NaOH and aliquot into tubes or flasks. Sterilise in the autoclave at 121℃ for 15 minutes (the S&H media with Sucrose should be autoclaved at 115℃ for 20 minutes or might turn dark at over 115℃).
^ During preparation, avoid naked flames and do not mix the basal salt mixture with reducing substances vigorously.
Storage
Media powder is extremely hygroscopic and should be protected from atmospheric moisture. If possible, the unused powder should be stored in a sealed box with silica desiccant and refrigerated at 2-8°C.
* Please select the product via the 'Media Type' drop-down box and purchase
** To request a quote and order a customised version, please contact support@gmexpression.com
For more pack sizes, products, and international purchases, please visit our shop at https://shop.gmexpression.com