Yeast Synthetic Drop-out Medium, without His-Met (Yeast double Defective Medium, SD-Dropout Medium, SD-His-Met Medium, SD-HM)

Cautions

This media is prepared in small batches and will be prepared after the purchase, taking around 5 to 10 business days for handling.

Product Description:

• Yeast SD-His-Met Defective Medium, also known as SD-HM Medium, is a synthetic drop-out medium for screening nutritionally-deficient Saccharomyces cerevisiae for use in yeast protein expression, yeast hybrid, double-hybrid system, and so on.
• SD deficient medium lack of corresponding components compared to yeast complete medium, which results in yeast strains that cannot synthesize the corresponding components and not being able to grow in the related defective type of medium.
• *This product does not contain sugar, and users can add glucose, raffinose or galactose according to the purpose of the experiment.

Use

• SD-HM  is used to screen for nutritionally deficient Saccharomyces cerevisiae.
• SD-HM  can be used as a protein expression medium for Saccharomyces cerevisiae when galactose is added.
• SD-HM added with glucose can be used as a medium for the enrichment of defective yeast.
• Since the composition of media is known, it can be used to identify nutritionally deficient types of Saccharomyces cerevisiae.

Storage:  kept in a cool and dry place.

Product properties: light yellow powder. when in the form of liquid: light amber, transparent, no precipitation or slight precipitation (insoluble calcium sulfate).

Components / per litre

Yeast base nitrogen source (YNB) 1.7g

Ammonium sulfate 5g

L-Arginine 0.1g

L-Cysteine 0.1g

L-Lysine 0.1g

L-Threonine 0.1g

L-Asparagine 0.05g

L-Isoleucine 0.05g

L-Phenylalanine 0.05

L-Proline 0.05g

L-Serine 0.05g

L-Tyrosine 0.05g

L-Valine 0.05g

L-Tryptophan 0.1g

L-Leucine 0.1g

Adenine 0.1g

Uridine 0.1g

Preparation

[Fast preparation] 

First, stir the powder in bottle B well with a spoon or glass rod* and take out 1.15 g of mixture B, suspend with 6.7 g of mixture A (SC medium), plus a suitable amount of sugar** in distilled or Milli-Q Water, make up to 1000ml.

Sterilise by autoclaving at 121℃ for 15 minutes***. Aliquot as needed.

*  Do not mix by shaking, as this will cause the lysine (red particles), which is much lighter than the other substances, to settle on the top of the mixture.

**  10g recommended

***  If equipment allows, autoclave at 115℃ for 30 minutes to prevent the darkening of the solution due to a Maillard reaction on sugar catalysed by ammonium sulphate and heat. 

[Standard preparation]  

1. Prepare 0.5 litre of 10X SC stock solution:

Dissolve 33.5 g mixture A with a suitable amount of sugar**/*** in distilled or Milli-Q water and mix well, make up to 500 ml.  Sterilise by 0.22µm filtration (recommended) or autoclave at 115℃ for 30 minutes.  Store the stock at room temperature. The shelf life of this solution is approximately one year.

2. Stir the powder in bottle B well with a spoon or glass rod* and take out 1.15 g of mixture B, suspend in distilled or Milli-Q Water, make up to 900ml. Sterilise by autoclaving at 121°C for 15 minutes.

3 Add 100 mL of sterile 10X SD stock into the freshly autoclaved (no longer boiling) mixture B solution, mix well and pour the plates while hot.  Store the PAD agar plates at 4°C. The shelf life is several months.

*  Do not mix by shaking, as this will cause the lysine (red particles), which is much lighter than the other substances, to settle on the top of the mixture.

**  50g recommended

*** Pre-prepared sugar stock, like 20% dextrose stock (Sterilised by 0.22µm filtration) also works.

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GMES sells and customises a variety of yeast SD-Dropout media. The catalogues are listed below, and customers can request a quote and order less commonly used SD-Dropout media via support@gmexpression.com

Yeast SD Defective Medium （corresponding defective component is removed from the complete medium formulation) 

SD complete medium

SD One Defective Medium.

• SD-Ura medium
• SD-His medium
• SD-Leu medium
• SD-Met medium
• SD-Ade medium
• SD-Trp medium

SD Double Defective Medium.

• SD-Ura-His medium
• SD-Ura-Leu Medium