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sodium chloride 10.0 g/L
tryptone (vegetable) 10.0 g/L
yeast extract 5.0 g/L
Final pH 7.0 ± 0.1 at 25 ℃
Preparation
Suspend 25.0 g of LB Broth (or with Agar for preparing solid media) in distilled water, mix well and make up to 1000 ml.
Sterilise by autoclaving at 121℃ for 15 minutes. Aliquot as needed.