The second assay used to confirm a positive HIV ELISA result is the Western Blot. Students separate protein samples from hypothetical patients on agarose gels, transfer the samples to a membrane and detect the simulated HIV proteins. This kit is an introductory level experiment. For a comprehensive advanced course, we recommend Cat. #317.
Includes:
Instructions, samples, standard molecular weight markers, protein agarose, various buffers and reagents, PVDF membrane, filter paper, stain, 1 ml pipet, 100 ml graduated cylinder.
Features:
• Understand the theory and applications of Western blots
• Learn the molecular biology of HIV and the pathogenesis of AIDS
• Perform electrophoresis and a modified western blot to detect simulated viral proteins
• Easy set up using standard (horizontal) electrophoresis equipment
• For 6 Blots
Manual:
https://www.edvotek.com/site/pdf/275.pdf