SEPARATION

GE Cytiva AKTApure 25 M Chromatography System | Protein Purification

Modular benchtop FPLC chromatography system for fast purification of proteins, peptides, and nucleic acids at laboratory scale.

QC PASS Tested & Working

At a glance

Manufacturer

GE Healthcare / Cytiva

Model

ÄKTA pure 25 M

Fraction Collector F9-C

Part / Catalog #

29018226

29015408

Serial #

2208444

2200996

Year / DOM

2017

Condition

Used - Excellent

What's Included

GE Cytiva ÄKTA pure 25 M chromatography system
Fraction Collector F9-C
Dell desktop computer with Unicorn 7.6 software
Power cords, Ethernet cable
UniNet-9 control cable
Nothing else is included unless shown in the photos

Condition & Testing

Unit powers on and UNICORN software loads to a Ready state with both system pumps, all valves, and the U9-M UV monitor recognized; no cracks or damage; flow-path calibration and performance qualification are the responsibility of the buyer.

Specifications

ÄKTA pure 25 M — Main Unit
System type	Laboratory-scale FPLC / liquid chromatography purification system
Configured modules	V9-Inj, V9-IAB, V9-M, V9-L, V9-C, V9-pH, V9-O, U9-M, C9, L9, F9-C, P9 A, P9 B, R9, M9
System pumps	Two high-performance pumps (P9 A and P9 B), one pair of pump heads each
Flow rate range	0.001–25 mL/min (normal); up to 50 mL/min for column packing
Max operating pressure	200 bar (2900 psi, 20 MPa)
UV monitor	U9-M multi-wavelength; 190–700 nm, up to 3 wavelengths simultaneously
UV flow cell path lengths	0.5, 2 (included at delivery), and 10 mm
UV lamp	High-intensity xenon lamp; typical operating time ~5000 h; auto-calibrated at power-on
Conductivity monitor	C9 (in-line, for gradient verification)
System pressure monitor	R9 (continuous; auto-adjusts flow to avoid pressure limits)
Mixer	M9; 1.4 mL chamber included (optional 0.6 and 5 mL); in-line filter mounted inside
Injection valve	V9-Inj; supports sample loops, Superloops, and direct-to-column application
Sample loop	500 µL delivered with system
Inlet valve	V9-IAB (selection between two A and two B positions)
Column valve	V9-C (built-in bypass and reverse-flow functionality)
pH valve	V9-pH (in-line pH monitoring, calibration, and storage)
Outlet valve	V9-O
External air sensor	L9 (column protection)
Chromatography techniques	Affinity, size exclusion (SEC), ion exchange, hydrophobic interaction, and reversed phase (RPC)
Wetted materials	Biocompatible; resistant to commonly used solvents
Power input	100–240 VAC, 50/60 Hz
Power consumption	1100 VA (max)
Mains fuse	T10AL250V
Mains out	As mains in (2 A max)
Communication	UniNet-9 instrument interface; Ethernet (IP 10.1.1.1)
Ingress protection	IP21
Weight	48 kg basic configuration; 53 kg fully equipped
Fraction Collector F9-C
Model	F9-C flexible fraction collector
Code no.	29015408
Serial no.	2200996
Year / DOM	2017/02
Collection format	Deep-well plates and/or tubes
Outlet positions	10 outlet positions + waste
Voltage	32 V via UniNet-9 interface
Power	18 VA max
Ingress protection	IP21

Specs from manufacturer literature for reference. Verify against your application.

Terms & Conditions

Payment

We accept most payment types and work with your organization's accounting requirements.
Contact us if you need a formal quote or to procure with a PO.

Shipping

Items ship within 1 to 2 business days after cleared payment.
We ship via USPS, FedEx, UPS, or freight, to the contiguous USA and worldwide.
Worldwide buyers are responsible for customs and duty import charges.
Need expedited service or billing to your own FedEx or UPS account? Tell us at purchase.
You may arrange your own shipping through your own carrier on request.

Return Policy

60-day returns on all products from time of delivery.
If anything is not right, contact us first. We want you fully satisfied.

Why BioLabTek

We power on and function-test, then document what we saw
Photos show the exact unit you receive
Instruments packed to survive the carrier
POs and formal quotes welcome for academic and corporate buyers