This authentic used Eppendorf (formerly New Brunswick) cell-lift sparger assembly is engineered for integration into bioreactor systems, specifically compatible with BioFlo models (e.g., BioFlo 3000, BioFlo 320) and CelliGen series (e.g., CelliGen 115, CelliGen 310), accommodating vessel sizes from 1 L to 14 L. It is optimized for microcarrier-based cell culture applications, facilitating low-shear gas delivery to enhance oxygen transfer efficiency while minimizing cellular damage in processes such as vaccine production, monoclonal antibody development, and stem cell research.

Key Features:

• Constructed from 316L electro-polished stainless steel for biocompatibility and autoclavability.
• Central sintered filter (porous element with approximately 10-20 micron pores) wrapped around the inner column for fine bubble diffusion.
• Protective aeration cage with mesh slots (approximately 75-80 micron openings) to safeguard microcarriers and prevent clogging.
• Top gas intake port with three radial side ports for multi-gas mixing (e.g., air, O₂, CO₂, N₂) or overlay aeration.
• Improves volumetric mass transfer coefficient (kLa) at reduced gas flow rates, supporting batch, fed-batch, perfusion, or continuous cultures.

Usage Guidelines:

• Mounts through the bioreactor headplate and integrates with the impeller shaft for optional rotation, reducing fouling in mammalian, insect, or anchorage-dependent cell lines (e.g., Vero, HEK293).
• Connects to thermal mass flow controllers for precise gas regulation; suitable for low-shear environments to achieve high cell densities (up to 8 × 10^6 cells/mL).
• Ideal for laboratory-scale R&D or pilot production in biopharmaceutical and biofuel sectors.

Specifications (Confirmed with Provided Measurements):

• Overall height: 260 mm (10.24 in)
• Chamber diameter: 75 mm (2.95 in)
• Three top outlets outer diameter: 19.05 mm (0.75 in)
• Intake diameter: 9.25 mm (0.36 in)
• Main chamber height: 127 mm (5.00 in)

These dimensions align with components designed for mid-range vessel capacities (e.g., 5-10 L) in Eppendorf/New Brunswick systems, confirming its identity as a protected sparger for microcarrier applications.