NovoCyte Benchtop Flow Cytometer – High-Performance Multi-Parameter Analysis by Agilent

The Agilent NovoCyte 1000 is a high-precision, benchtop flow cytometer ideal for streamlined multicolor analysis in both research and core labs. With a powerful 488 nm blue laser and three fluorescence detection channels, it delivers robust single-laser performance through an intuitive, automation-friendly design.Its advanced fluidics—featuring syringe-driven volumetric counting, active clog detection, and automated cleaning—ensure consistent performance and data quality. Adjustable flow rates (5–120 µL/min), a 7.2-log dynamic range, and full inter-beam compensation (enabled by NovoExpress software) enable sensitive, multivariate analysis with minimal hands-on time.Compact and capable, the NovoCyte 1000 integrates seamlessly into high-efficiency workflows, supporting both tube-based processing and automated sampling with its versatile sample format compatibility. Whether for routine immunophenotyping or research applications, it offers sensitive detection, efficient operation, and exceptional data integrity in a simple, space-saving package.Core Technical SpecificationsLaser & Detector ConfigurationEquipped with a single blue laser (488 nm).Provides three fluorescence channels for multicolor detection.Automated Compatibility & WorkflowBuilt for automation, enabling hands-off operation and integration with sample handlers.Supports 1.5 mL tubes, 12×75 mm tubes, and is automation-compatible for plate handling.Enhanced Sensitivity & RangeCapable of detecting up to 17 parameters with enhanced sensitivity and refined resolution, depending on configuration.Fluidics & Sample HandlingFlow Rate Flexibility: Adjust sample flow continuously from 5 µL/min to 120 µL/min, enabling fine control over throughput and resolution.Absolute Volumetric Counting: Uses a positive-displacement syringe pump for precise, bead-free absolute counts.Carryover Control: Automated cleaning routines ensure <0.1% carryover between samples.Fluidics Monitoring & Maintenance: Active sensors monitor pressure and fluid levels, enabling automated clog detection, system recovery, and comprehensive startup/shutdown cleaning—minimizing manual oversight.Optics & Detection PerformanceFlow Cell Design: Features a rectangular quartz flow cell measuring 170 × 290 µm, delivering stable optical alignment without user adjustments.Dynamic Range & Data Quality: Scoring 24-bit resolution (7.2-log dynamic range) with user-adjustable fluorescence detector gains, it minimizes the need for manual tuning.Captures height and area parameters for FSC, SSC, and fluorescence, as well as width (FSC) and time, for robust data acquisition.Compensation & Reporting: Supports full interbeam compensation, both during and post-acquisition; outputs in FCS 3.0/3.1, CSV, and PDF, complete with customizable batch reporting.Software & Data WorkflowPowered by Agilent NovoExpress software, offering intuitive acquisition, analysis, and automated reporting.Compatible with a standard workstation (Intel i7, 8 GB RAM, 1 TB HDD); higher configurations available when needed.Why the NovoCyte 1000 ShinesCompact & User-Friendly: A modest footprint with automation support fits seamlessly into tight lab spaces.Scalable Throughput: Adjustable flow rates and automated fluidics make it ideal for both low-volume research and higher-demand screening.High Data Integrity with Minimal Effort: Wide dynamic detection, auto-compensation, and stable optics ensure reproducible, high-quality data with minimal intervention.Efficient Maintenance & Operation: From automated cleaning to fluid level monitoring, daily operation is simplified and reliable.