Electrophoresis Station Bio-Rad Experion

The Experion™ automated electrophoresis system employs LabChip microfluidic technology to automate protein and nucleic acid electrophoresis. It integrates separation, detection, and data analysis within a single platform. Using much smaller sample and reagent quantities than standard analysis method, the Experion system accomplishes analysis in a single 30–40 minute, automated step.

 

Electrophoresis Station

The Experion automated electrophoresis station houses all the electrical, optical, and hardware components necessary to perform the separation, staining, destaining, detection, and imaging of samples in a single step. The electrophoresis station is equipped with an internal universal power supply that operates at 100–240 VAC, 50–60 Hz. It connects to a computer (PC) running the Experion software.

 

Applications of Experion System

The Experion system is used for the following applications:

• Protein analysis — quantitation and size determination (sizing) of protein sample useful for quality control, protein purity and stability analysis, protocol optimization, and evaluation of recombinant protein expression
• RNA analysis — evaluation of RNA integrity and concentration. The system automatically generates the RNA quality indicator (RQI), a quantitative integrity assessment, in addition to visual electropherogram data, ribosomal RNA ratios, and concentration data. This makes Experion RNA assays useful upstream of applications such as quantitative real-time PCR (qPCR), microarray analysis, sequencing, and reverse-transcription qPCR (RT-qPCR)
• DNA analysis — quantitation and sizing of restriction digests, amplified DNA, microsatellites, and amplified fragment length polymorphisms (AFLPs).