The early days of bluegrass saw performers adhering to a strict - if unspoken - law of 'men only.' A few brave women challenged that rule, lighting the way for generations of female musicians to follow. It has only been in the past few decades that women have been received on their own terms, and bluegrass has grown enormously as a result. Rounder Records has proudly supported female bluegrass artists throughout its thirty year existence, and artists like Claire Lynch, Lynn Morris, Alison Krauss, and Hazel Dickens have emerged as some of the most popular, successful, and respected musicians in modern roots music. With one unreleased gem joining selections from Rounder's large library of classic music, O Sister! is a revelation: the first compilation to thoroughly explore women's vast contribution to this unique American art.
| Track | Artist | Title | Composer | |
| 01 | Laurie Lewis | Tall Pines | Damon Black | |
| 02 | Lynn Morris | It Rains Everywhere I Go | Don Cusic | |
| 03 | Alecia McRight | I'll Stay Around | Lester Flatt | |
| 04 | Jeannie Kendall | I Wonder Where You Are Tonight | Johnny Bond | |
| 05 | Suzanne Thomas | Leaving The Land | R. Williasm, L. Williams | |
| 06 | Rhonda Vincent | Pretending I Don't Care | Jimmy Martin, Paul M. Williams | |
| 07 | Ginny Hawker | Don't Neglect The Rose | Emma Smith | |
| 08 | Hazel Dickens | Love Me Or Leave Me Alone | R. Bryson | |
| 09 | Kathy Kallick With Laurie Lewis | Hideaway | Debbie Cotter | |
| 10 | The Stevens Sisters | Who's Crying For You Now | Betty Stevens, Beth Stevens | |
| 11 | Tanya Savory | Reason Enough | Tanya Savory | |
| 12 | Claire Lynch | Hey Lonesome | Jimmy Martin, Sam Humphrey | |
| 13 | Carol Elizabeth Jones With James Leva | Someday | Carol Elizabeth Jones | |
| 14 | Hazel Dickens And Alice Gerrard | Montana Cowboy | Jack Sutton | |
| 15 | Olabelle Reed | High On A Mountain | Olabelle Reed, Alex Campbell | |
| 16 | Wilma Lee Cooper | Poor Ellen Smith | Traditional, Arr. Wilma Lee Cooper, Stoney Cooper | |
| 17 | Ginny Hawker And Hazel Dickens | Times Are Not What They Used To Be | Traditional, Arr. Dickins, Hawker | |
| 18 | Phyllis Boyens With Hazel Dickens | Dream Of A Miner's Child | ||
| 19 | Wayfaring Strangers Featuring Lucy Kaplansky And Jennifer Kimball | Memories Of You | James B. Smith | |
| 20 | Marie Burns With The Woodshed All-Stars | Willow | Martie Burns, Martin Simpson | |
| 21 | Alison Krauss And Suzanne Cox |