The Leica PL FLUOTAR 40x/0.70 ∞/0.17/D (Article/Part Number: 506004)
is a professional-grade microscope objective lens designed for research-grade
Leica infinity-corrected microscope systems (such as the DMR and DM series).
Optical and Technical
Specifications
- PL FLUOTAR (Semi-Apochromat): "PL" stands for Plan,
indicating excellent field flatness across the entire field of view,
making it ideal for both visual observation and digital imaging.
"FLUOTAR" specifies that it utilizes fluorite glass
formulations. These provide superior chromatic and spherical aberration
correction compared to standard Achromat objectives, delivering highly
accurate color rendering and excellent contrast.
- 40x Magnification:
Provides a medium-to-high magnification level, suitable for observing
cellular structures, histology sections, and microorganisms.
- 0.70 Numerical Aperture (NA): The NA of 0.70 dictates the light-gathering
capacity and resolving power of the objective. For a dry 40x objective,
0.70 is a high numerical aperture, offering a resolving power down to
approximately 0.4 microns under standard visible light.
- ∞ (Infinity Corrected): This symbol signifies that the objective is
designed for an infinity-corrected optical system. Light leaves the
objective as parallel rays, allowing optical components (such as sliders,
filters, or beamsplitters) to be inserted into the parallel light path
without introducing optical aberrations.
- 0.17:
Indicates that the objective is optically corrected for use with a
standard 0.17 mm thick glass coverslip (No. 1.5). Deviations from
this coverslip thickness can cause spherical aberration and degrade image
sharpness.
- D (Dry / DIC Compatibility): * The "D" stands for Dry,
meaning it operates with air between the objective tip and the coverslip
(no immersion oil or water required).
- In Leica's naming convention for this era, the
letter classification (specifically D) also indicates its
compatibility group for Differential Interference Contrast (DIC).
It determines which specific Wollaston/Nomarski prism (often designated
as a "K" or "D" type prism in the condenser/slider)
matches the objective's back focal plane to achieve proper DIC contrast.
Mechanical Properties
- Threading:
Typically utilizes Leica’s standard M25 x 0.75 metric thread
pattern, which is wider than standard RMS threading.
- Parfocal Distance:
Designed to match Leica's standard 45mm parfocal distance, meaning
the sample remains mostly in focus when switching between this and other
calibrated Leica objectives on the nosepiece turret.
Common Applications
Due to its high contrast, color correction, and flat field, this objective
is commonly used in:
- Transmitted light brightfield
microscopy.
- Fluorescence microscopy (due to the high
UV/visible light transmission properties of Fluotar glass).
- Differential Interference Contrast (DIC)
microscopy.