Luminol 10g
ultrapure, >99,9%
Luminol is used by forensic investigators to detect trace amounts of blood left at crime scenes , as it reacts with iron found in hemoglobin. It is used by biologists in cellular assays for the detection of copper , iron , and cyanides , as well as of specific proteins by western blot .
To exhibit its luminescence, the luminol must be activated with an oxidant. Usually, a solution containing hydrogen peroxide (H2O2) and hydroxide ions in water is used as the activator. In a laboratory setting, the catalyst used is often potassium ferricyanide:
In a transparent container with 200-500 mL of water was added 1-3 g of luminol and 1-2 g of sodium hydroxide (or potassium hydroxide), the contents are mixed well. Thereafter, in the dark was added 100-150 ml of 5% hydrogen peroxide. Begins a faint glow. Next added a pinch of crystal potassium ferricyanide. Starts strong luminescence.
It is possible to create a glow during the oxidation of luminol with oxygen from the air. In a transparent container mix 25 g of crushed potassium hydroxide 0.1 g luminol and 100ml of dimethyl sulfoxide (DMSO). Blue luminescence appears with stirring.