Product Description
The E638 15390 DNA Polymerase α-Primase includes both RNA primase and DNA polymerase subunits. This enzyme is essential for synthesizing a longer than twenty base hybrid RNA-DNA primer. The DNA Pol1 subunit does not feature a 3-prime to 5-prime proofreading exonuclease, which allows it to efficiently traverse common oxidized or hydrolyzed template nucleotides often found during chromosome replication.
- Eukaryotic Polα-primase synthesizes a >20-nucleotide hybrid RNA-DNA primer using DNA polymerase (Pol1) and RNA primase subunits.
- Pol1 lacks a proofreading 3-prime to 5-prime exonuclease, enabling it to navigate various template lesions produced by hydrolytic and metabolic oxidative environments.
- Polα is uniquely effective at navigating common oxidized or hydrolyzed template nucleotides, indicating an evolutionary adaptation to bypass these lesions during chromosome replication.
Product Highlights
The DNA Polymerase α-Primase synthesizes RNA-DNA primers and efficiently traverses template lesions, with support from replication factor C (RFC) to stimulate Polα lesion bypass.
Product Information
Standard Information
- Keywords: DNA polymerase alpha, DNA translesion bypass, primase, DNA replication, DNA repair
- Published in: Proceedings of the National Academy of Sciences
Technical Specifications
- Volume: 122
- Issue Number: 37
- Published Online: September 10, 2025
- Published in Issue: September 16, 2025
- Received: July 2, 2025
- Accepted: August 14, 2025
- PubMed ID: 40928879
- Classifications: Biological Sciences, Biochemistry